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Article

Plasmid construction using recombination activity in the fission yeast Schizosaccharomyces pombe.

PloS one - 11 Mar 2010

Chino Ayako, Watanabe Kenji, Moriya Hisao

Abstract excerpt

BACKGROUND: Construction of plasmids is crucial in modern genetic manipulation. As of now, the common method for constructing plasmids is to digest specific DNA sequences with restriction enzymes and to ligate the resulting DNA fragments with DNA ligase. Another potent method to construct plasmids, known as gap-repair cloning (GRC), is commonly used in the budding yeast Saccharomyces cerevisiae. GRC makes use of...

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Identifiers

PMID: 20300182
DOI: 10.1371/journal.pone.0009652
PMCID: PMC2836380

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