Article

Isolation of Full Size BAC Inserts by DNA Gap Repair in <i>E. coli</i>

2019-02-14

Abstract excerpt

DNA polymers can comprise millions of base pairs and encode thousands of structural and regulatory genetic elements. Thus, the precise isolation of specific DNA segments is required for accurate gene dissection. Although polymerase chain reaction (PCR) is a standard tool for this purpose, increasing DNA template size leads to the accumulation of polymerase errors, hindering the precise isolation of large-size DNA...

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Literature Corpus work: a16335e4-9594-509a-b962-eea340afc98c
DOI: 10.1101/549634

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