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Article

Experimental analysis of sources of error in evolutionary studies based on Roche/454 pyrosequencing of viral genomes.

Genome biology and evolution - 1 Jan 2012

Becker Ericka A, Burns Charles M, León Enrique J, Rajabojan Saravanan, Friedman Robert, Friedrich Thomas C, O'Connor Shelby L, Hughes Austin L

Abstract excerpt

Factors affecting the reliability of Roche/454 pyrosequencing for analyzing sequence polymorphism in within-host viral populations were assessed by two experiments: 1) sequencing four clonal simian immunodeficiency virus (SIV) stocks and 2) sequencing mixtures in different proportions of two SIV strains with known fixed nucleotide differences. Observed nucleotide diversity and frequency of undetermined...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Simian Acquired Immunodeficiency Syndrome

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Identifiers

PMID: 22436995
DOI: 10.1093/gbe/evs029
PMCID: PMC3342875

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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