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Article

A Systematic Evaluation of High-Throughput Sequencing Approaches to Identify Low-Frequency Single Nucleotide Variants in Viral Populations.

Viruses - 20 Oct 2020

King David J, Freimanis Graham, Lasecka-Dykes Lidia, Asfor Amin, Ribeca Paolo, Waters Ryan, King Donald P, Laing Emma

Abstract excerpt

High-throughput sequencing such as those provided by Illumina are an efficient way to understand sequence variation within viral populations. However, challenges exist in distinguishing process-introduced error from biological variance, which significantly impacts our ability to identify sub-consensus single-nucleotide variants (SNVs). Here we have taken a systematic approach to evaluate laboratory and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33092085
DOI: 10.3390/v12101187
PMCID: PMC7594041

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