Article
A comparison of parallel pyrosequencing and sanger clone-based sequencing and its impact on the characterization of the genetic diversity of HIV-1.
PloS one - 1 Jan 2011
Liang Binhua, Luo Ma, Scott-Herridge Joel, Semeniuk Christina, Mendoza Mark, Capina Rupert, Sheardown Brent, Ji Hezhao, Kimani Joshua, Ball Blake T, Van Domselaar Gary, Graham Morag, Tyler Shane, Jones Steven J M, Plummer Francis A
Abstract excerpt
BACKGROUND: Pyrosequencing technology has the potential to rapidly sequence HIV-1 viral quasispecies without requiring the traditional approach of cloning. In this study, we investigated the utility of ultra-deep pyrosequencing to characterize genetic diversity of the HIV-1 gag quasispecies and assessed the possible contribution of pyrosequencing technology in studying HIV-1 biology and evolution....
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