Article
Dominant negative umuD mutations decreasing RecA-mediated cleavage suggest roles for intact UmuD in modulation of SOS mutagenesis.
Proceedings of the National Academy of Sciences of the United States of America - 1 Sept 1990
Battista J R, Ohta T, Nohmi T, Sun W, Walker G C
Abstract excerpt
The products of the SOS-regulated umuDC operon are required for most UV and chemical mutagenesis in Escherichia coli. The UmuD protein shares homology with a family of proteins that includes LexA and several bacteriophage repressors. UmuD is posttranslationally activated for its role in mutagenesis by a RecA-mediated proteolytic cleavage that yields UmuD'. A set of missense mutants of umuD was isolated and shown...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Codon
- Coliphages
- DNA Repair
- DNA-Directed DNA Polymerase
- Escherichia coli
- Escherichia coli Proteins
- Genes, Dominant
- Molecular Sequence Data