Article
Escherichia coli umuDC mutants: DNA sequence alterations and UmuD cleavage.
Molecular & general genetics : MGG - 1 Jun 1992
Koch W H, Ennis D G, Levine A S, Woodgate R
Abstract excerpt
The products of the chromosomally encoded umuDC genes are directly required for mutagenesis in Escherichia coli. Strains with either umuD or umuC mutations are rendered phenotypically non-mutable. To ascertain the molecular basis of this non-mutability, we determined the DNA sequence alterations of seven chromosomal umuDC mutants. Six mutants (umuD1, umuD44, umuD77, umuC36, umuC25, and umuC104) were found to be...
Topics
- Bacterial Proteins
- Base Sequence
- DNA, Bacterial
- DNA-Directed DNA Polymerase
- Escherichia coli
- Escherichia coli Proteins
- Immunoassay
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Polymerase Chain Reaction