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Article

Inducible cassette exchange: a rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells.

Stem cells (Dayton, Ohio) - 1 Oct 2011

Iacovino Michelina, Bosnakovski Darko, Fey Holger, Rux Danielle, Bajwa Gagan, Mahen Elisabeth, Mitanoska Ana, Xu Zhaohui, Kyba Michael

Abstract excerpt

Genetic modification is critically enabling for studies addressing specification and maintenance of cell fate; however, methods for engineering modifications are inefficient. We demonstrate a rapid and efficient recombination system in which an inducible, floxed cre allele replaces itself with an incoming transgene. We target this inducible cassette exchange (ICE) allele to the (HPRT) locus and demonstrate...

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Identifiers

PMID: 22039605
DOI: 10.1002/stem.715
PMCID: PMC3622722

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