Article
Efficient allele conversion in mouse zygotes and primary cells based on electroporation of Cre protein.
Methods (San Diego, Calif.) - 1 Jul 2021
Jenickova Irena, Kasparek Petr, Petrezselyova Silvia, Elias Jan, Prochazka Jan, Kopkanova Jana, Navratil Michal, Barinka Cyril, Sedlacek Radislav
Abstract excerpt
Cre-loxP recombination system is a powerful tool for genome engineering. One of its applications is found in genetic mouse models that often require to induce Cre recombination in preimplantation embryos. Here, we describe a technically simple, affordable and highly efficient protocol for Cre protein delivery into mouse zygotes by electroporation. We show that electroporation based delivery of Cre has no negative...
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