Article
RMCE-ASAP: a gene targeting method for ES and somatic cells to accelerate phenotype analyses.
Nucleic acids research - 26 Jul 2006
Toledo Franck, Liu Chung-Wen, Lee Crystal J, Wahl Geoffrey M
Abstract excerpt
In recent years, tremendous insight has been gained on p53 regulation by targeting mutations at the p53 locus using homologous recombination in ES cells to generate mutant mice. Although informative, this approach is inefficient, slow and expensive. To facilitate targeting at the p53 locus, we developed an improved Recombinase-Mediated Cassette Exchange (RMCE) method. Our approach enables efficient targeting in...
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