Article

Efficient CRISPR/Cas9-mediated homology independent sequence replacement in vivo and non-dividing primary cells

2026-07-24

Abstract excerpt

Precise sequence replacement in non-dividing cells remains a major challenge for genome editing. Here we show that REPLACE (Rational end-joining protocol delivering a targeted sequence exchange), a homology-independent CRISPR/Cas9-based replacement strategy, enables exon- and gene-scale substitution in primary cells, in vivo tissues and post-mitotic human cardiomyocytes. REPLACE uses two guide RNAs to excise a de...

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Literature Corpus work: 92703db4-64b3-5348-b827-ff0e4fafe1f6
DOI: 10.64898/2026.07.24.740048

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