Article
Functional interaction among catalytic residues in subtilisin BPN'.
Proteins - 1 Jan 1990
Carter P, Wells J A
Abstract excerpt
Variants of the serine protease, subtilisin BPN', in which the catalytic triad residues (Ser-221, His-64, and Asp-32) are replaced singly or in combination by alanine retain activities with the substrate N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide (sAAPF-pna) that are at least 10(3) to 10(4...
Topics
- Alanine
- Amino Acid Sequence
- Bacillus
- Base Sequence
- Binding Sites
- Hydrogen Bonding
- Kinetics
- Molecular Sequence Data
- Mutation
- Ovomucin
- Subtilisins