Article
Rendering one autolysis site in Bacillus subtilis neutral protease resistant to cleavage reveals a new fission.
Biotechnology and applied biochemistry - 1 Apr 1998
Van den Burg B, Eijsink V G, Vriend G, Veltman O R, Venema G
Abstract excerpt
Autolytic degradation of the thermolysin-like proteinase of Bacillus subtilis (TLP-sub) is responsible for the irreversible inactivation of the enzyme at elevated temperatures. Previously we have reported five cleavage sites in TLP-sub [Van den Burg et al. (1990) Biochem. J. 272, 93-97]. In an at...
Topics
- Amino Acid Sequence
- Bacillus subtilis
- Binding Sites
- Enzyme Stability
- Metalloendopeptidases
- Models, Molecular
- Mutation
- Protein Conformation
- Sequence Analysis
- Substrate Specificity