Article
Rendering one autolysis site in Bacillus subtilis neutral protease resistant to cleavage reveals a new fission.
Biotechnology and applied biochemistry - 1 Apr 1998
Van den Burg B, Eijsink V G, Vriend G, Veltman O R, Venema G
Abstract excerpt
Autolytic degradation of the thermolysin-like proteinase of Bacillus subtilis (TLP-sub) is responsible for the irreversible inactivation of the enzyme at elevated temperatures. Previously we have reported five cleavage sites in TLP-sub [Van den Burg et al. (1990) Biochem. J. 272, 93-97]. In an attempt to render the enzyme less susceptible to autolytic breakdown, one of the fission sites, located between Leu-156...
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