Article
Crystal structures of subtilisin BPN' variants containing disulfide bonds and cavities: concerted structural rearrangements induced by mutagenesis.
Proteins - 1 Jan 1990
Katz B, Kossiakoff A A
Abstract excerpt
The X-ray structures of four genetically engineered disulfide variants of subtilisin have been analyzed to determine the energetic and structural constraints involved in inserting disulfide bonds into proteins. Each of the engineered disulfides exhibited atypical sets of dihedral angles compared with known structures of natural disulfide bridges in proteins and affected its local structural environment to a...
Topics
- Base Sequence
- Chemical Phenomena
- Chemistry, Physical
- Disulfides
- Models, Molecular
- Molecular Sequence Data
- Molecular Structure
- Mutation
- Protein Conformation
- Subtilisins
- X-Ray Diffraction