Article
Isolation of a dnaA mutant of Bacillus subtilis defective in initiation of replication: amount of DnaA protein determines cells' initiation potential.
The EMBO journal - 1 Sept 1990
Moriya S, Kato K, Yoshikawa H, Ogasawara N
Abstract excerpt
The dnaA gene is essential for initiation of chromosomal replication in Escherichia coli. A gene homologous with the E. coli dnaA was found in the replication origin region of the Bacillus subtilis chromosome. We have now isolated a temperature sensitive mutant of the B. subtilis dnaA by in vitro mutagenesis of the cloned gene. At a nonpermissive temperature, 49 degrees C, DNA replication stops completely after...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- Blotting, Western
- Cloning, Molecular
- DNA Replication
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Sequence Homology, Nucleic Acid