Article
Hyperinitiation of DNA replication in Escherichia coli leads to replication fork collapse and inviability.
Molecular microbiology - 1 Jan 2004
Simmons Lyle A, Breier Adam M, Cozzarelli Nicholas R, Kaguni Jon M
Abstract excerpt
Elevated dnaA expression from a multicopy plasmid induces more frequent initiation from the Escherichia coli replication origin, oriC, but viability is maintained. In comparison, chromosomally encoded dnaAcos also stimulates initiation, but this is lethal. By quantitative methods, we show that the level of initiation induced by elevated dnaA expression leads to collapsed replication forks that are mostly within...
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