Article
Escherichia coli DnaA protein: specific biochemical defects of mutant DnaAs reduce initiation frequency to suppress a temperature-sensitive dnaX mutation.
Biochimie - 1 Jan 2006
Walker James R, Severson Kimberly Ann, Hermandson Mary Jo, Blinkova Alexandra, Carr Kevin M, Kaguni Jon M
Abstract excerpt
The Escherichia coli dnaA73, dnaA721, and dnaA71 alleles, which encode A213D, R432L, T435K substitutions, respectively, were originally isolated as extragenic suppressors of a temperature-sensitive dnaX mutant. As the A213D substitution resides in a domain that functions in ATP binding and the R432L and T435K substitutions affect residues that recognize the DnaA box motif, they might be expected to reduce ATP and...
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