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Article

Purification and characterization of an initiation protein for chromosomal replication, DnaA, in Bacillus subtilis.

Journal of biochemistry - 1 May 1990

Fukuoka T, Moriya S, Yoshikawa H, Ogasawara N

Abstract excerpt

Bacillus subtilis DnaA protein was overproduced by a recombinant plasmid containing B. subtilis dnaA gene in a mutant Escherichia coli strain which is deficient in its own DnaA and RNaseH. The protein was purified to near homogeneity as judged by SDS-PAGE analysis. The purified protein binds preferentially to DNA fragments which are derived from flanking regions of the B. subtilis dnaA gene and contain various...

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PMID: 2168872
DOI: 10.1093/oxfordjournals.jbchem.a123117

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Related research

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Purification and characterization of an initiation protein for chromosomal replication, DnaA, in Bacillus subtilis.PMID 2168872Read ↗

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The DNA binding domain of the initiator protein DnaA.Semantic proximity Read ↗

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Research on DnaA in the early days.Semantic proximity Read ↗

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