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Article

Mutagenesis of Thr-286 in monomeric Ca2+/calmodulin-dependent protein kinase II eliminates Ca2+/calmodulin-independent activity.

Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1990

Waxham M N, Aronowski J, Westgate S A, Kelly P T

Abstract excerpt

We have examined the role of Thr-286 autophosphorylation in the autoregulation of Ca2+/calmodulin-dependent protein kinase II. Using site-directed mutagenesis, we have substituted alanine or serine for Thr-286, or isoleucine for Arg-283, in the 50-kDa subunit of the kinase and expressed each protein in bacteria. Activation and autophosphorylation of all four enzymes were stringently dependent on Ca2+/calmodulin,...

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Source

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Identifiers

PMID: 2154738
DOI: 10.1073/pnas.87.4.1273
PMCID: PMC53456

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Source receipt: pubmed, retrieved 7 Sept 2026.

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