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Article

Mutagenesis of Thr-286 in monomeric Ca2+/calmodulin-dependent protein kinase II eliminates Ca2+/calmodulin-independent activity.

Proceedings of the National Academy of Sciences of the United States of America - 1 Feb 1990

Waxham M N, Aronowski J, Westgate S A, Kelly P T

Abstract excerpt

We have examined the role of Thr-286 autophosphorylation in the autoregulation of Ca2+/calmodulin-dependent protein kinase II. Using site-directed mutagenesis, we have substituted alanine or serine for Thr-286, or isoleucine for Arg-283, in the 50-kDa subunit of the kinase and expressed each protein in bacteria. Activation and autophosphorylation of all four enzymes were stringently dependent on Ca2+/calmodulin,...

Topics

Arginine
Calcium
Calcium-Calmodulin-Dependent Protein Kinases
Calmodulin
Cloning, Molecular
Isoleucine
Kinetics
Macromolecular Substances
Mutation
Phosphorylation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Kinases

Recombinant Proteins

Threonine

Source

Open in PubMed Open full text

Identifiers

PMID: 2154738
DOI: 10.1073/pnas.87.4.1273
PMCID: PMC53456

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2154738-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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