Regulation-defective mutants of type I cAMP-dependent protein kinase. Consequences of replacing arginine 94 and arginine 95.

Article

Regulation-defective mutants of type I cAMP-dependent protein kinase. Consequences of replacing arginine 94 and arginine 95.

The Journal of biological chemistry - 5 Aug 1993

Buechler Y J, Herberg F W, Taylor S S

Abstract excerpt

The type I alpha regulatory subunits of cAMP-dependent protein kinase contain an autoinhibitor site, Arg94-Arg-Gly-Ala-Ile, which serves as a pseudosubstrate. To evaluate their contribution to subunit association, Arg94 and Arg95, key determinants for peptide recognition, were replaced singly and...

Topics

Amino Acid Sequence
Arginine
Chromatography, Gel
Cyclic AMP
Kinetics
Molecular Sequence Data
Mutation
Protein Conformation
Protein Kinases

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