Studies on the regulatory domain of Ca2+/calmodulin-dependent protein kinase II by expression of mutated cDNAs in Escherichia coli.

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Article

Studies on the regulatory domain of Ca2+/calmodulin-dependent protein kinase II by expression of mutated cDNAs in Escherichia coli.

The Journal of biological chemistry - 5 Sept 1991

Hagiwara T, Ohsako S, Yamauchi T

Abstract excerpt

The cDNAs encoding the alpha and beta subunits of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) were ligated into the bacterial expression vector pET and expressed in Escherichia coli. The bacterially expressed alpha and beta subunits exhibited Ca2+/calmodulin-dependent activity and were easily purified to apparent homogeneity from cell extracts. To determine the minimum size required for catalytic...

Topics

Amino Acid Sequence
Animals
Base Sequence
Calcium
Calcium-Calmodulin-Dependent Protein Kinases
Calmodulin
Catalysis
Cloning, Molecular
DNA, Bacterial
Electrophoresis, Polyacrylamide Gel
Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutagenesis

Mutation

Phosphorylation

Protein Kinases

Temperature

Source

Open in PubMed

Identifiers

PMID: 1653235

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1653235-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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