Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Expression and characterization of the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II using the baculovirus expression system.

Biochemical and biophysical research communications - 14 Dec 1990

Brickey D A, Colbran R J, Fong Y L, Soderling T R

Abstract excerpt

Sf9 cells infected with the recombinant mouse CaMKII-alpha (Ca2+/calmodulin dependent kinase II) baculovirus expressed 12-15 mg of MCaMKII-alpha per liter of cells. Approximately 50% of the MCaMKII-alpha activity could be purified using a CaM-Sepharose affinity column. The purified MCaMKII-alpha had a M(rapp) of 50 kDa by SDS-PAGE and a native Mr of 600 kDa. MCaMKII-alpha, like rat brain CaMKII, had an A0.5 for...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cells, Cultured
Share to Gene Expression

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Kinetics

Share to Mutation

Share to Phosphorylation

Share to Recombinant Proteins

Source

Identifiers

PMID: 2175600
DOI: 10.1016/s0006-291x(05)80074-9

Genes

SDS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Expression and characterization of the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II using the baculovirus expression system.PMID 2175600Read ↗

Methods Optimization for the Expression and Purification of Human Calcium Calmodulin-Dependent Protein Kinase II AlphaSemantic proximity Read ↗

Studies on the regulatory domain of Ca2+/calmodulin-dependent protein kinase II by expression of mutated cDNAs in Escherichia coli.Semantic proximity Read ↗

CaMKIIβ in Neuronal Development and Plasticity: An Emerging Candidate in Brain Diseases.Semantic proximity Read ↗

Identification and characterization of delta B-CaM kinase and delta C-CaM kinase from rat heart, two new multifunctional Ca2+/calmodulin-dependent protein kinase isoforms.Semantic proximity Read ↗

The pseudokinase CaMKv is required for the activity-dependent maintenance of dendritic spines.Semantic proximity Read ↗

Activation mechanisms for Ca2+/calmodulin-dependent protein kinase IV. Identification of a brain CaM-kinase IV kinase.Semantic proximity Read ↗

Overexpression, purification, and characterization of human m-calpain and its active site mutant, m-C105S-calpain, using a baculovirus expression system.Semantic proximity Read ↗

Human calcium-calmodulin dependent protein kinase I: cDNA cloning, domain structure and activation by phosphorylation at threonine-177 by calcium-calmodulin dependent protein kinase I kinase.Semantic proximity Read ↗

Calcium/calmodulin-dependent protein kinase I in Xenopus laevis.Semantic proximity Read ↗

In vivo and in vitro characterization of the sequence requirement for oligomer formation of Ca2+/calmodulin-dependent protein kinase IIalpha.Semantic proximity Read ↗

Role of beta isoform-specific insertions of Ca2+/calmodulin-dependent protein kinase II.Semantic proximity Read ↗

New isoforms of Ca2+/calmodulin-dependent protein kinase II in smooth muscle.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.