Article
Expression and characterization of the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II using the baculovirus expression system.
Biochemical and biophysical research communications - 14 Dec 1990
Brickey D A, Colbran R J, Fong Y L, Soderling T R
Abstract excerpt
Sf9 cells infected with the recombinant mouse CaMKII-alpha (Ca2+/calmodulin dependent kinase II) baculovirus expressed 12-15 mg of MCaMKII-alpha per liter of cells. Approximately 50% of the MCaMKII-alpha activity could be purified using a CaM-Sepharose affinity column. The purified MCaMKII-alpha...
Topics
- Animals
- Baculoviridae
- Brain
- Calcium-Calmodulin-Dependent Protein Kinases
- Cells, Cultured
- Gene Expression
- Kinetics
- Mice
- Mutation
- Phosphorylation
- Protein Kinases
- Rats
- Recombinant Proteins