Article
Evidence that autophosphorylation at Thr-286/Thr-287 is required for full activation of calmodulin-dependent protein kinase II.
Biochimica et biophysica acta - 28 May 1996
Ishida A, Kitani T, Fujisawa H
Abstract excerpt
Calmodulin-dependent protein kinase II (CaM-kinase II) undergoes a very rapid autophosphorylation at Thr-286/Thr-287 in the presence of Ca2+/calmodulin and ATP/Mg2+, and this has greatly hampered studies on the role of the autophosphorylation in the regulation of the enzyme activity, because it h...
Topics
- Adenosine Triphosphate
- Animals
- Brain
- Calcium
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Intercellular Signaling Peptides and Proteins
- Kinetics
- Mutation
- Peptides
- Phosphates
- Phosphorylation
- Rats
- Recombinant Proteins
- Threonine