Article
Dissecting the domain structure of the regulatory subunit of cAMP-dependent protein kinase I and elucidating the role of MgATP.
The Journal of biological chemistry - 25 Mar 1990
Ringheim G E, Taylor S S
Abstract excerpt
A truncated regulatory subunit of cAMP-dependent protein kinase I was constructed which contained deletions at both the carboxyl terminus and at the amino terminus. The entire carboxyl-terminal cAMP-binding domain was deleted as well as the first 92 residues up to the hinge region. This monomeric...
Topics
- Adenosine Triphosphate
- Amino Acid Sequence
- Animals
- Cattle
- Chromosome Deletion
- Cyclic AMP
- Enzyme Activation
- Genetic Vectors
- Kinetics
- Macromolecular Substances
- Molecular Sequence Data
- Mutation
- Protein Binding
- Protein Kinases
- Recombinant Proteins