Article
Systematic mutational analysis of cAMP-dependent protein kinase identifies unregulated catalytic subunits and defines regions important for the recognition of the regulatory subunit.
The Journal of biological chemistry - 5 Mar 1992
Gibbs C S, Knighton D R, Sowadski J M, Taylor S S, Zoller M J
Abstract excerpt
A library of mutants of the catalytic subunit of the Saccharomyces cerevisiae cAMP-dependent protein kinase was screened in vitro for mutants defective in the recognition of the regulatory subunit. The mutations identified were mapped onto the three-dimensional structure of the mouse catalytic su...
Topics
- Amino Acid Sequence
- Catalysis
- Kinetics
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Oligopeptides
- Plasmids
- Protein Kinases
- Regulatory Sequences, Nucleic Acid
- Saccharomyces cerevisiae
- Sequence Homology, Nucleic Acid