Reconstitution of normal and hyperactivated forms of casein kinase-2 by variably mutated beta-subunits.

Abstract excerpt

Twenty-one mutants of the noncatalytic beta-subunit of human casein kinase-2 have been created, expressed in Escherichia coli, and purified to homogeneity. They are either modified at the autophosphorylation site (mutants beta delta 1-4 and beta A 5,6) or bear variable deletions in their C-terminal part (mutants beta delta 209-215, beta delta 194-215, beta delta 181-215, beta delta 171-215, beta delta 150-215) or...

Topics

• Amino Acid Sequence
• Casein Kinases
• Catalysis
• Cloning, Molecular
• Enzyme Activation
• Genetic Variation
• Heparin
• Humans
• Kinetics
• Molecular Sequence Data
• Mutagenesis
• Polymerase Chain Reaction