Article
Crystallographic analyses illustrate significant plasticity and efficient recoding of meganuclease target specificity.
Nucleic acids research - 21 Aug 2017
Werther Rachel, Hallinan Jazmine P, Lambert Abigail R, Havens Kyle, Pogson Mark, Jarjour Jordan, Galizi Roberto, Windbichler Nikolai, Crisanti Andrea, Nolan Tony, Stoddard Barry L
Abstract excerpt
The retargeting of protein-DNA specificity, outside of extremely modular DNA binding proteins such as TAL effectors, has generally proved to be quite challenging. Here, we describe structural analyses of five different extensively retargeted variants of a single homing endonuclease, that have been shown to function efficiently in ex vivo and in vivo applications. The redesigned proteins harbor mutations at up to...
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