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Article

A unified genetic, computational and experimental framework identifies functionally relevant residues of the homing endonuclease I-BmoI.

Nucleic acids research - 1 Apr 2010

Kleinstiver Benjamin P, Fernandes Andrew D, Gloor Gregory B, Edgell David R

Abstract excerpt

Insight into protein structure and function is best obtained through a synthesis of experimental, structural and bioinformatic data. Here, we outline a framework that we call MUSE (mutual information, unigenic evolution and structure-guided elucidation), which facilitated the identification of previously unknown residues that are relevant for function of the GIY-YIG homing endonuclease I-BmoI. Our approach...

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Identifiers

PMID: 20061372
DOI: 10.1093/nar/gkp1223
PMCID: PMC2853131

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