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Article

The I-TevI nuclease and linker domains contribute to the specificity of monomeric TALENs.

G3 (Bethesda, Md.) - 16 Apr 2014

Kleinstiver Benjamin P, Wang Li, Wolfs Jason M, Kolaczyk Tomasz, McDowell Brendon, Wang Xu, Schild-Poulter Caroline, Bogdanove Adam J, Edgell David R

Abstract excerpt

Precise genome editing in complex genomes is enabled by engineered nucleases that can be programmed to cleave in a site-specific manner. Here, we fused the small, sequence-tolerant monomeric nuclease domain from the homing endonuclease I-TevI to transcription-like activator effectors (TALEs) to create monomeric Tev-TALE nucleases (Tev-mTALENs). Using the PthXo1 TALE scaffold to optimize the Tev-mTALEN...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24739648
DOI: 10.1534/g3.114.011445
PMCID: PMC4065259

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