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Article

Progressive engineering of a homing endonuclease genome editing reagent for the murine X-linked immunodeficiency locus.

Nucleic acids research - 1 Jun 2014

Wang Yupeng, Khan Iram F, Boissel Sandrine, Jarjour Jordan, Pangallo Joseph, Thyme Summer, Baker David, Scharenberg Andrew M, Rawlings David J

Abstract excerpt

LAGLIDADG homing endonucleases (LHEs) are compact endonucleases with 20-22 bp recognition sites, and thus are ideal scaffolds for engineering site-specific DNA cleavage enzymes for genome editing applications. Here, we describe a general approach to LHE engineering that combines rational design with directed evolution, using a yeast surface display high-throughput cleavage selection. This approach was employed to...

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Identifiers

PMID: 24682825
DOI: 10.1093/nar/gku224
PMCID: PMC4041414

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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