Article
Association between the cellular p53 and the adenovirus 5 E1B-55kd proteins reduces the oncogenicity of Ad-transformed cells.
The EMBO journal - 1 Aug 1990
van den Heuvel S J, van Laar T, Kast W M, Melief C J, Zantema A, van der Eb A J
Abstract excerpt
The association of the cellular p53 protein with the E1B-55kd protein of adenovirus 5 (Ad5) is thought to result in inactivation of the p53 recessive oncogene product. Here we show that Ad5 E1-transformed 3Y1 rat cells which express low levels of the 55 kd E1B protein do not contain the p53-E1B 55kd complex. These cells have nuclearly located p53 and are highly oncogenic in nude mice. In 3Y1 cells expressing the...
Topics
- Adenovirus Early Proteins
- Adenoviruses, Human
- Animals
- Antibodies, Monoclonal
- Blotting, Western
- Cell Line
- Cell Transformation, Neoplastic
- Cytotoxicity, Immunologic
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel