Adenovirus E1B 55-kilodalton protein is a p53-SUMO1 E3 ligase that represses p53 and stimulates its nuclear export through interactions with promyelocytic leukemia nuclear bodies.

Abstract excerpt

Oncogenic transformation by adenovirus E1A and E1B-55K requires E1B-55K inhibition of p53 activity to prevent E1A-induced apoptosis. During viral infection, E1B-55K and E4orf6 substitute for the substrate-binding subunits of the host cell cullin 5 class of ubiquitin ligases, resulting in p53 polyubiquitinylation and proteasomal degradation. Here we show that E1B-55K alone also functions as an E3 SUMO1-p53 ligase....

Topics

• Active Transport, Cell Nucleus
• Adenovirus E1B Proteins
• Cell Line, Tumor
• Cell Transformation, Neoplastic
• Dimerization
• Humans
• Intranuclear Inclusion Bodies
• Microscopy, Fluorescence
• Models, Biological
• Multiprotein Complexes
• Mutation
• Nuclear Proteins