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Article

Role of vacuolar acidification in protein sorting and zymogen activation: a genetic analysis of the yeast vacuolar proton-translocating ATPase.

Molecular and cellular biology - 1 Jul 1990

Yamashiro C T, Kane P M, Wolczyk D F, Preston R A, Stevens T H

Abstract excerpt

Vacuolar acidification has been proposed to play a key role in a number of cellular processes, including protein sorting, zymogen activation, and maintenance of intracellular pH. We investigated the significance of vacuolar acidification by cloning and mutagenizing the gene for the yeast vacuolar proton-translocating ATPase 60-kilodalton subunit (VAT2). Cells carrying a vat2 null allele were viable; however,...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Enzyme Activation
Enzyme Precursors
Escherichia coli
Genes, Fungal
Genotype
Hydrogen-Ion Concentration
Macromolecular Substances

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Oligonucleotide Probes

Proton-Translocating ATPases

Restriction Mapping

Saccharomyces cerevisiae

Sequence Homology, Nucleic Acid

Vacuoles

Source

Open in PubMed Open full text

Identifiers

PMID: 2141385
DOI: 10.1128/mcb.10.7.3737-3749.1990
PMCID: PMC360825

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2141385-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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