Article
Genes required for vacuolar acidity in Saccharomyces cerevisiae.
Genetics - 1 Jul 1992
Preston R A, Reinagel P S, Jones E W
Abstract excerpt
Mutations that cause loss of acidity in the vacuole (lysosome) of Saccharomyces cerevisiae were identified by screening colonies labeled with the fluorescent, pH-sensitive, vacuolar labeling agent, 6-carboxyfluorescein. Thirty nine vacuolar pH (Vph-) mutants were identified. Four of these contained mutant alleles of the previously described PEP3, PEP5, PEP6 and PEP7 genes. The remaining mutants defined eight...
Topics
- Alleles
- Fluoresceins
- Genes, Fungal
- Genetic Complementation Test
- Hydrogen-Ion Concentration
- Mutagenesis
- Saccharomyces cerevisiae
- Vacuoles