Article
[Screening for K-ras mutations in colorectal and lung cancers by using a novel real-time PCR with ADx-K-ras kit and Sanger DNA sequencing].
Zhonghua bing li xue za zhi = Chinese journal of pathology - 1 Nov 2010
Zhang Hai-Ping, Fu Li, Chen Pei-Qiong, Ye Yun-Bin, Ji Tian-Hai, Zheng Li-Mou
Abstract excerpt
OBJECTIVE: to map out the frequency and types of K-ras gene mutations present in colorectal and lung cancer patients; to evaluate the clinical applicability of a novel real-time double-loop probe PCR using the ADx-K-ras kit, and to compare its performance with the result by using traditional Sanger DNA sequencing in detection of somatic mutations of the tumor genes. METHODS: a total of 827 formalin-fixed...
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