Article
Comparative screening of K-ras mutations in colorectal cancer and lung cancer patients using a novel real-time PCR with ADx-K-ras kit and Sanger DNA sequencing.
Cell biochemistry and biophysics - 1 Apr 2012
Zhang Haiping, Zheng Xiongwei, Ji Tianhai, Fu Li, Bai Dongyu, Liao Yongqiang, Zhang Haifang, Ding Yi, Zheng Limou
Abstract excerpt
We determined frequency/types of K-ras mutations in colorectal/lung cancer. ADx-K-ras kit (real-time/double-loop probe PCR) was used to detect somatic tumor gene mutations compared with Sanger DNA sequencing using 583 colorectal and 244 lung cancer paraffin-embedded clinical samples. Genomic DNA was used in both methods; mutation rates at codons 12/13 and frequency of each mutation were detected and compared. The...
Topics
- Colorectal Neoplasms
- Gene Frequency
- Genetic Testing
- Humans
- Lung Neoplasms
- Mutation
- Mutation Rate
- Reagent Kits, Diagnostic
- Real-Time Polymerase Chain Reaction
- Sequence Analysis, DNA
- ras Proteins