Article
[K-ras gene mutation in colorectal cancer and its clinicopathologic significance].
Zhonghua wai ke za zhi [Chinese journal of surgery] - 15 Aug 2010
Yuan Ying, Hu Han-guang, Ye Xiao-xian, Shen Hong, Zheng Shu
Abstract excerpt
OBJECTIVE: To establish a simple, rapid and economical method in detecting mutations of oncogene K-ras and to investigate its mutations in colorectal cancer tissues and its relationship with clinicopathologic characteristics of colorectal carcinoma. METHODS: Forty colorectal cancer tissues were tested for K-ras mutations at codon 12 and codon 13 using polymerase chain reaction (PCR) followed by direct sequencing...
Topics
- Adult
- Aged
- Aged, 80 and over
- Colorectal Neoplasms
- Female
- Genes, ras
- Humans
- Male
- Middle Aged
- Mutation
- Polymorphism, Restriction Fragment Length
