Article
Point mutations of c-ras genes in human bladder cancer and kidney cancer.
Japanese journal of cancer research : Gann - 1 Jan 1990
Nagata Y, Abe M, Kobayashi K, Saiki S, Kotake T, Yoshikawa K, Ueda R, Nakayama E, Shiku H
Abstract excerpt
Point mutations of c-ras genes at codons 12, 13 and 61 were analyzed in 26 cases of bladder cancer and 16 cases of kidney cancer. DNA prepared from either frozen tissues or 10% formalin-fixed, paraffin-embedded tissues were amplified by means of polymerase chain reaction methods, and mutations were analyzed by dot blot hybridization assays with oligonucleotide probes. In three cases of bladder cancer c-ras...
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