Detection of a rare point mutation in Ki-ras of a human bladder cancer xenograft by polymerase chain reaction and direct sequencing.

Abstract excerpt

This paper represents the first report of a codon 59 mutation in Ki-ras from a spontaneous human transitional cell carcinoma of the bladder. Point mutations have the potential to activate the ras genes if they occur in critical coding regions. These include the sequences of codons 12, 13, 59, 61 and 63. Mutations in codons 12, 13 and 61 have been reported in a wide variety of human cancers, including transitional...

Topics

• Animals
• Base Sequence
• Blotting, Northern
• Carcinoma, Transitional Cell
• Codon
• DNA Mutational Analysis
• Genes, ras
• Humans
• Immunoblotting
• Mice
• Mice, Inbred BALB C