Article
Multiplex polymerase chain reaction amplification and direct sequencing of homologous sequences: point mutation analysis of the ras genes.
Analytical biochemistry - 15 Nov 1991
Manam S, Nichols W W
Abstract excerpt
ras proto-oncogenes are activated by point mutation in a wide variety of human and animal tumors, making ras gene analysis a major area of clinical and basic cancer research. Activating point mutations, in each of the three ras genes (Ha-, Ki-, or N-ras), usually occur in one of three specific co...
Topics
- Animals
- Base Sequence
- Codon
- DNA
- DNA, Neoplasm
- DNA-Directed DNA Polymerase
- Genes, ras
- Liver
- Liver Neoplasms
- Mice
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction
- Sequence Homology, Nucleic Acid
- Taq Polymerase