Article
Screening of human bladder tumors and urine sediments for the presence of H-ras mutations.
International journal of cancer - 11 Nov 1993
Levesque P, Ramchurren N, Saini K, Joyce A, Libertino J, Summerhayes I C
Abstract excerpt
A series of 111 human bladder tumors were screened using oligonucleotide mutant specific probes, restriction enzyme analysis and single-stranded confirmation polymorphism (SSCP) for the presence of H-ras activation events. Thirty-three tumors were found to harbor H-ras mutations where a glycine t...
Topics
- 3T3 Cells
- Animals
- Base Sequence
- Blotting, Southern
- Cell Line
- DNA, Neoplasm
- DNA, Single-Stranded
- Genes, ras
- Humans
- Mice
- Molecular Sequence Data
- Mutation
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Transfection
- Urinary Bladder Neoplasms