Article
Kdo2-lipid A, a TLR4-specific agonist, induces de novo sphingolipid biosynthesis in RAW264.7 macrophages, which is essential for induction of autophagy.
The Journal of biological chemistry - 3 Dec 2010
Sims Kacee, Haynes Christopher A, Kelly Samuel, Allegood Jeremy C, Wang Elaine, Momin Amin, Leipelt Martina, Reichart Donna, Glass Christopher K, Sullards M Cameron, Merrill Alfred H
Abstract excerpt
Activation of RAW264.7 cells with a lipopolysaccharide specific for the TLR4 receptor, Kdo(2)-lipid A (KLA), causes a large increase in cellular sphingolipids, from 1.5 to 2.6 × 10(9) molecules per cell in 24 h, based on the sum of subspecies analyzed by "lipidomic" mass spectrometry. Thus, this study asked the following question. What is the cause of this increase and is there a cell function connected with it?...
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