Article
Rapid confirmation of gene targeting in embryonic stem cells using two long-range PCR techniques.
Transgenic research - 1 Mar 1998
Lay J M, Friis-Hansen L, Gillespie P J, Samuelson L C
Abstract excerpt
Gene targeting in mouse embryonic stem (ES) cells generally includes the analysis of numerous colonies to identify a few with mutations resulting from homologous recombination with a targeting vector. Thus, simple and efficient screening methods are needed to identify targeted clones. Optimal screening approaches require probes from outside of the region included in the targeting vector to avoid detection of the...
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