Rapid confirmation of gene targeting in embryonic stem cells using two long-range PCR techniques.

doi:10.1023/a:1008876526826

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Article

Rapid confirmation of gene targeting in embryonic stem cells using two long-range PCR techniques.

Transgenic research - 1 Mar 1998

Lay J M, Friis-Hansen L, Gillespie P J, Samuelson L C

Abstract excerpt

Gene targeting in mouse embryonic stem (ES) cells generally includes the analysis of numerous colonies to identify a few with mutations resulting from homologous recombination with a targeting vector. Thus, simple and efficient screening methods are needed to identify targeted clones. Optimal scr...

Topics

Alleles
Animals
Cholecystokinin
Cloning, Molecular
Embryo, Mammalian
Gastrins
Gene Targeting
Mice
Polymerase Chain Reaction
Sequence Analysis, DNA
Stem Cells

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