Article
Engineering the mouse genome with bacterial artificial chromosomes to create multipurpose alleles.
Nature biotechnology - 1 Apr 2003
Testa Giuseppe, Zhang Youming, Vintersten Kristina, Benes Vladimir, Pijnappel W W M Pim, Chambers Ian, Smith Andrew J H, Smith Austin G, Stewart A Francis
Abstract excerpt
The mouse is the leading vertebrate model because its genome can be altered by both random transgenesis and homologous recombination with targeting constructs. Both approaches have been hindered by the size and site limitations implicit in conventional Escherichia coli DNA-engineering methods. Homologous recombination in E. coli, or 'recombineering', has overcome these limitations for bacterial artificial...
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