Article
Structural identification of recombinant human CD83 mutant variant as a potent therapeutic protein.
Protein expression and purification - 1 Oct 2010
Zhang Lin, Narayanan Niju, Brand Stephen R, Nicolette Charles A, Baroja Miren, Arp Jacqueline, Wang Hao, Moo-Young Murray, Chou C Perry
Abstract excerpt
The formation of aberrant disulfide bonds is a structural consideration for the manufacturing of the extracellular domain of human CD83 (hCD83ext), a potential therapeutic protein. In certain instances, hCD83ext protein products, even when stored frozen, tended to dimerize or even multimerize through the formation of aberrant intermolecular disulfide bonds. Herein, we discovered an analytical inconsistency and...
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