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Article

Substituting the Thiol Ester of Human A2M or C3 with a Disulfide Produces Native Proteins with Altered Proteolysis-Induced Conformational Changes.

Biochemistry - 29 Dec 2020

Harwood Seandean Lykke, Nielsen Nadia Sukusu, Pedersen Henrik, Kjøge Katarzyna, Nielsen Peter Kresten, Andersen Gregers Rom, Enghild Jan J

Abstract excerpt

Most proteins in the α-macroglobulin (αM) superfamily contain reactive thiol esters that are required for their biological function. Here, we have characterized the human α2-macroglobulin (A2M) and complement component C3 mutants A2M Q975C and C3 Q1013C, which replace the CGEQ thiol ester motifs of the original proteins with the disulfide-forming sequence CGEC. Mass spectrometry showed that the intended disulfide...

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PMID: 33301305
DOI: 10.1021/acs.biochem.0c00803

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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