Article
Molecular manipulation associated with disulfide bond formation to enhance the stability of recombinant therapeutic protein.
Protein expression and purification - 1 Jan 2011
Zhang Lin, Moo-Young Murray, Chou C Perry
Abstract excerpt
Cys²⁷ in the extracellular domain of human CD83 (hCD83ext), a potential therapeutic protein, was identified as a target for molecular manipulation. Two Escherichia coli strains of BL21(DE3) and Origami B(DE3), respectively, with a reducing and an oxidative cytoplasm were used as the expression host to produce the Cys²⁷ mutants. It was observed that Cys²⁷ was involved in the in vivo formation of intramolecular...
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