Characterization of genetic variation and 3'-azido-3'-deoxythymidine- resistance mutations of human immunodeficiency virus by the RNase A mismatch cleavage method.

doi:10.1073/pnas.88.10.4280

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Article

Characterization of genetic variation and 3'-azido-3'-deoxythymidine- resistance mutations of human immunodeficiency virus by the RNase A mismatch cleavage method.

Proceedings of the National Academy of Sciences of the United States of America - 15 May 1991

López-Galíndez C, Rojas J M, Nájera R, Richman D D, Perucho M

Abstract excerpt

The RNase A mismatch cleavage method has been applied to the characterization of natural genetic variation of human immunodeficiency virus (HIV) from different geographical areas. The approach provides a rapid and simple assay for the analysis of differences in closely related viral isolates and allows the establishment of phylogenetic relationships between epidemiologically distinct viruses. Our results show a...

Topics

Acquired Immunodeficiency Syndrome
Base Sequence
Codon
DNA, Viral
Drug Resistance, Microbial
Genes, Viral
Genetic Variation
HIV
HIV Envelope Protein gp120
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Nucleic Acid Hybridization

Polymerase Chain Reaction

Ribonuclease, Pancreatic

Viral Proteins

Zidovudine

Source

Open in PubMed Open full text

Identifiers

PMID: 2034672
DOI: 10.1073/pnas.88.10.4280
PMCID: PMC51642

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2034672-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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