Article
HIV-1 sensitivity to zidovudine: a consensus culture technique validated by genotypic analysis of the reverse transcriptase.
Journal of virological methods - 1 May 1992
Brun-Vézinet F, Ingrand D, Deforges L, Gochi K, Ferchal F, Schmitt M P, Jung M, Masquelier B, Aubert J, Buffet-Janvresse C
Abstract excerpt
In order to select and standardize a reliable assay for the analysis of sensitivity of HIV isolates to AZT, we have compared two culture methods. The first assay (Cell-Associated Isolate Sensitivity Assay: CAISA) quantified AZT-resistant HIV isolates by end-point dilution cultures of peripheral b...
Topics
- Cell-Free System
- Culture Media
- Genotype
- HIV Reverse Transcriptase
- HIV-1
- Humans
- Kinetics
- RNA-Directed DNA Polymerase
- Virus Replication
- Zidovudine