Article
The 3'-azido group is not the primary determinant of 3'-azido-3'-deoxythymidine (AZT) responsible for the excision phenotype of AZT-resistant HIV-1.
The Journal of biological chemistry - 12 Aug 2005
Sluis-Cremer Nicolas, Arion Dominique, Parikh Urvi, Koontz Dianna, Schinazi Raymond F, Mellors John W, Parniak Michael A
Abstract excerpt
The mechanism of human immunodeficiency virus (HIV) 1 resistance to 3'-azido-3'-deoxythymidine (AZT) involves reverse transcriptase (RT)-catalyzed phosphorolytic excision of the chain-terminating AZT-5'-monophosphate (AZTMP). Primers terminated with AZTMP are generally better substrates for this reaction than those terminated with 2',3'-dideoxynucleoside-5'-monophosphate (2',3'-ddNMP) analogs that lack a 3'-azido...
Topics
- Anti-HIV Agents
- Binding Sites
- Catalysis
- Cell Line
- DNA
- DNA Primers
- Drug Resistance, Viral
- HIV-1
- Humans
- Kinetics
- Mutation
- Phenotype
- Regression Analysis
- T-Lymphocytes